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The laboratory of
Dr. Decho (1750 sq. ft.) is located on the 5th floor of
the Public Health
Research Center (PHRC) Building in the Arnold School of Public Health. Our laboratory is designed
to conduct microbial biofilm research.
Virtual Lab Tour
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>> Click rooms for images |
Our instrumentation includes:
- Microbial culture room equipped HEPA-filtered
air filtration & temperature/light incubators.
- Scanning Laser confocal microscopy and quantitative imaging
capabilities (a new Leica TCS SP5 Confocal System with Broadband,
AOBS, FLIM, FRET, FRAP; and an Olympus System: Fluoview IX70
Inverted Scope w/Kr-Ar excitation lasers and DIC);
- COBRA II AutoGamma Photon Counting System (Packard);
- TriCarb 2300TR Liquid Scintillation Counting System (Packard);
- Nexus 670 Fourier Tranform Infared Spectrometer (FT-IR) equipped
for single & multibounce ATR;
- Fluoromax II Spectrofluorometer (Jobin-SPEX);
- Shimadzu UV-2401 UV-VIS Spectrophotometer;
- PAGE (1-D and 2-D) Gel Systems;
- Veritas Microplate Luminometer;
- (two lyophilizers, balances, hoods, water purification systems,
autoclaves, ultracold freezers, etc); Our capabilities include
fluorescence in-situ hybridization (FISH)
capabilities; quorum sensing signal characterization; various
molecular approaches; Image analysis/processing;
- a new Raman/Confocal Microscopy Facility (Bruker Senterra System, Dept. ENHS
* We also have access to:
- The new
USC Environmental Genomics Facility (in Dept. ENHS,
Arnold School of Public Health);
- The USC Microscopy Center which houses a (1) a Single and
2-Photon Scanning laser microscopy system (BioRad MRC 1024MP
equipped with a Nikon Eclipse TE 300 Inverted Compound Microscope);
TEM, SEM, ESEM.
- The
USC Medical School Microscopy Center
houses a (2) Zeiss LSM 510 META Confocal System on a Zeiss
Axioplan 200 motorized microscope, located at the USC Medical
School.
Lasers include a 405 diode, argon, and red
and green HeNe lasers providing a wide range of available excitation
values for selection of fluorochromes. This system is also equipped
for advanced imaging techniques such as spectral separation of
signals, FRET and FRAP.
- The
USC Mass Spectrometry,
Proteomics, and NMR Facilities house a range of instrumentation
including a
Finnigan 4500 - Quadrupole instrument, a
Micromass Q-TOF I - Tandem quadrupole-time of flight mass
spectrometer; a Micromass Quattro LC - Triple quadrupole mass spectrometer; a
Bruker Ultraflex MALDI TOF/TOF
- Matrix assisted laser
desorption/ ionization (MALDI) mass spectrometer and a
Micromass Q-TOF API-US - Highly sensitive hybrid quadrupole-time
of flight mass spectrometer configured for online nano-LC-MS/MS
analysis of protein mixtures, and a Varian Inova 500 mHz NMR.
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